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A rapid method for identifying the origin of North AmericanPhragmites populations using RFLP analysis

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Abstract

The common reed,Phragmites australis, is a common feature in wetlands across North America. Recent studies have suggested that the widespread invasions of this species may be due to the introduction of a non-native strain from EurAsia. Since native population types is needed to facilitate management of ulations are also found, a method for distinguishing population persist in many areas where introduced populations are also found, a method for distinguishing population types is needed to facilitate management of the species. A restriction fragment length polymorphism (RFLP) assay was developed to distinguish native, non-native, and Gulf Coast type populations ofPhragmites from each other. Two amplified non-coding chloroplast DNA regions are each cut with one restriction enzyme, allowing the distinction of native, non-native and Gulf Coast haplotypes from each other. When used together, these cut sites provide a low cost, rapid way of determining the origin ofPhragmites populations.

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  1. Kristin Saltonstall

    Present address: Horn Point Laboratory, University of Maryland Center for Environmental Science, PO Box 775, 21613, Cambridge, Maryland, USA

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  1. Department of Ecology and Evolutionary Biology, Yale University, PO Box 208106, 06520, New Haven, Connecticut, USA

    Kristin Saltonstall

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  1. Kristin Saltonstall
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Saltonstall, K. A rapid method for identifying the origin of North AmericanPhragmites populations using RFLP analysis. Wetlands 23, 1043–1047 (2003). https://doi.org/10.1672/0277-5212(2003)023[1043:ARMFIT]2.0.CO;2

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  • DOI: https://doi.org/10.1672/0277-5212(2003)023[1043:ARMFIT]2.0.CO;2

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